Free Flashcard From Module 4
the standard PCR method (in sufficient detail) and how this process is able to
produce clones in a "cell-free" system.
target DNA is denatured using heat, giving two single strands. Primers are
added to flank the DNA of interest, and extended using Taq DNA polymerase. The
result is two new pieces of whole DNA containing the targeted gene. This is
repeated 25-40 times to yield the desired amount of clones. Because all of
these cellular machinery can run in this artificial environment, it does not
require a host cell to proceed.