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Module 4

Question:

Describe the standard PCR method (in sufficient detail) and how this process is able to produce clones in a "cell-free" system.

Answer:

The target DNA is denatured using heat, giving two single strands. Primers are added to flank the DNA of interest, and extended using Taq DNA polymerase. The result is two new pieces of whole DNA containing the targeted gene. This is repeated 25-40 times to yield the desired amount of clones. Because all of these cellular machinery can run in this artificial environment, it does not require a host cell to proceed.
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Last update by mayur on 03/31/2014
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Module 4
Module 4
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Module 4, MG 500
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Question: Describe the standard PCR method (in sufficient detail) and how this process is able to produce clones in a "cell-free" system.
Answer: The target DNA is denatured using heat, giving two single strands. Primers are added to flank the DNA of interest, and extended using Taq DNA polymerase. The result is two new pieces of whole DNA containing the targeted gene. This is repeated 25-40 times to yield the desired amount of clones. Because all of these cellular machinery can run in this artificial environment, it does not require a host cell to proceed.