Module 4

Last update by mayur on 03/31/2014
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What is the purpose of an antibiotic resistance gene in a plasmid cloning vector?

Answer:
It is an effective selection system for bacteria.

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    What is the purpose of the LacZ gene in a plasmid cloning vector?
    It assists in differentiating bacterial plasmids with the specified target gene from those without it by dividing them into blue and white colonies.
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    Use the following list of markers found on a collection of cloned genomic fragments for this question.
    Image on third side.
    B, D, C, F
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    All of the following are characteristics of the genomics revolution EXCEPT____________.

    Large scale acquisition of DNA sequences
    Inability to understand single genes
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    Facilitated collaborative research networks
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    Which of the below are not steps in the production of genome sequence maps:

    Isolate whole chromosomes.
    Identify molecular markers on specific chromosomes.
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    Read the sequence of individual piece of the genome.
    When sequences are obtained, assemble and organize the sequences in order.
    Isolate the whole chromosome
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    Which technique would NOT be used to find a gene for a functional protein in a sequenced region of a genome?

    Scan the region for intron splice sites.
    See if an EST database contains sequences in the region.
    Scan the region for ORFs.
    Scan the region for promoter sequences.
    See if a SNP database contains sequences in the region.
    See if a SNP database contains sequences in the region.
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    What do PCR, reverse transcription, and dideoxy DNA sequencing all have in common?
    All produce DNA chains as a product.
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    Which of the following are the important proteins needed for cloning a eukaryotic gene into a bacterial plasmid?

    A)DNA polymerase
    B)restriction enzymes specific for the target genes
    C)DNA ligase
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    List two especially useful characteristics of cloning vectors.
    high copy number and antibiotic resistance gene(s)
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    There are different challenges that exist for sequencing prokaryotic and eukaryotic genomes. Which challenge is correctly paired with the type of genome to which it relates?
    Eukaryotic: repetitive DNA
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    What is bioinformatics?

    a method that uses very large national and international databases to access and work with sequence information
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    A typical prokaryotic genome has
    1 million base pairs of DNA, containing 1000 genes.