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Microbiology Lab Exam 2

Question:

Hydrolysis Reactions-


Tryptophan Hydrolysis

Answer:


Also known as "Indole" test.

Some bacteria can hydrolyze the amino acid tryptophan into pyruvate and indole.

The organism is inoculated into tryptone broth and allowed to incubate 24-48 hours at optimum temp.

Kovac's reagent is added to the tube after incubation.

A positive result= bright red ring at the surface of the broth
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Last update by bubabe12 on 10/24/2009
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Microbiology Lab Exam 2
Microbiology Lab Exam 2
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Question: Hydrolysis Reactions-Tryptophan Hydrolysis
Answer: Also known as "Indole" test.Some bacteria can hydrolyze the amino acid tryptophan into pyruvate and indole.The organism is inoculated into tryptone broth and allowed to incubate 24-48 hours at optimum temp.Kovac's reagent is added to the tube after incubation.A positive result= bright red ring at the surface of the broth