Chapter 20

Last update by mayur on 03/27/2014
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Which of the following cuts DNA molecules at specific locations?

A) restriction enzymes

B) gene cloning

C) DNA ligase

D) gel electrophoresis

E) reverse transcriptase

Answer:

A) restriction enzymes

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Related Quiz Content
  • feldman2009
    Answered in Chapter 20

    Which of the following separates molecules by movement due to size and electrical charge?

    A) restriction enzymes

    B) gene cloning

    C) DNA ligase

    D) gel electrophoresis

    E) reverse transcriptase

    D) gel electrophoresis

  • feldman2009
    Answered in Chapter 20

    Restriction fragments of DNA are typically separated from one another by which process?

    A) filtering

    B) centrifugation

    C) gel electrophoresis

    D) PCR

    E) electron microscopy

    C) gel electrophoresis

  • feldman2009
    Answered in Chapter 20

    In order to identify a specific restriction fragment using a probe, what must be done?

    A) The fragments must be separated by electrophoresis.

    B) The fragments must be treated with heat or chemicals to separate the strands of the double helix.

    C) The probe must be hybridized with the fragment.

    D) Only A and B are correct.

    E) A, B, and C are correct.

    E) A, B, and C are correct.
  • feldman2009
    Answered in Chapter 20

    Which of the following modifications is least likely to alter the rate at which a DNA fragment moves through a gel during electrophoresis?

    A) altering the nucleotide sequence of the DNA fragment

    B) methylating the cytosine bases within the DNA fragment

    C) increasing the length of the DNA fragment

    D) decreasing the length of the DNA fragment

    E) neutralizing the negative charges within the DNA fragment

    A) altering the nucleotide sequence of the DNA fragment

  • feldman2009
    Answered in Chapter 20

    DNA fragments from a gel are transferred to a nitrocellulose paper during the procedure called Southern blotting. What is the purpose of transferring the DNA from a gel to a nitrocellulose paper?

    A) to attach the DNA fragments to a permanent substrate

    B) to separate the two complementary DNA strands

    C) to transfer only the DNA that is of interest

    D) to prepare the DNA for digestion with restriction enzymes

    E) to separate out the PCRs

    A) to attach the DNA fragments to a permanent substrate

  • feldman2009
    Answered in Chapter 20

    RFLP analysis can be used to distinguish between alleles based on differences in which of the following?

    A) restriction enzyme recognition sites between the alleles

    B) the amount of DNA amplified from the alleles during PCR

    C) the ability of the alleles to be replicated in bacterial cells

    D) the proteins expressed from the alleles

    E) the ability of nucleic acid probes to hybridize to the alleles

    A) restriction enzyme recognition sites between the alleles

  • feldman2009
    Answered in Chapter 20

    Which of the following procedures would produce RFLPs?

    A) incubating a mixture of single-stranded DNA from two closely related species

    B) incubating DNA nucleotides with DNA polymerase

    C) incubating DNA with restriction enzymes

    D) incubating RNA with DNA nucleotides and reverse transcriptase

    E) incubating DNA fragments with "sticky ends" with ligase

    C) incubating DNA with restriction enzymes

  • feldman2009
    Answered in Chapter 20

    Dideoxyribonucleotide chain-termination is a method of

    A) cloning DNA.

    B) sequencing DNA.

    C) digesting DNA.

    D) synthesizing DNA.

    E) separating DNA fragments.

    B) sequencing DNA.

  • feldman2009
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    DNA microarrays have made a huge impact on genomic studies because they

    A) can be used to eliminate the function of any gene in the genome.

    B) can be used to introduce entire genomes into bacterial cells.

    C) allow the expression of many or even all of the genes in the genome to be compared at once.

    D) allow physical maps of the genome to be assembled in a very short time.

    E) dramatically enhance the efficiency of restriction enzymes.

    C) allow the expression of many or even all of the genes in the genome to be compared at once.

  • feldman2009
    Answered in Chapter 20

    Which was developed by a British researcher and causes DNA sequences to be transferred to a membrane and identified with a probe?

    A) Southern blotting

    B) Northern blotting

    C) Western blotting

    D) Eastern blotting

    E) RT-PCR

    A) Southern blotting

  • feldman2009
    Answered in Chapter 20

    Which describes the transfer of polypeptide sequences to a membrane to analyze gene expression?

    A) Southern blotting

    B) Northern blotting

    C) Western blotting

    D) Eastern blotting

    E) RT-PCR

    C) Western blotting