Chapter 20

Last update by mayur on 03/27/2014
491584 People have viewed this Quiz
  • Share

A principal problem with inserting an unmodified mammalian gene into a bacterial plasmid, and then getting that gene expressed in bacteria, is that

A) prokaryotes use a different genetic code from that of eukaryotes.

B) bacteria translate polycistronic messages only.

C) bacteria cannot remove eukaryotic introns.

D) bacterial RNA polymerase cannot make RNA complementary to mammalian DNA.

E) bacterial DNA is not found in a membrane-bounded nucleus and is therefore incompatible with mammalian DNA.

Answer:

C) bacteria cannot remove eukaryotic introns.

View Chapter 20 as Flashcard Deck

Related Quiz Content
  • feldman2009
    Answered in Chapter 20

    A gene that contains introns can be made shorter (but remain functional) for genetic engineering purposes by using

    A) RNA polymerase to transcribe the gene.

    B) a restriction enzyme to cut the gene into shorter pieces.

    C) reverse transcriptase to reconstruct the gene from its mRNA.

    D) DNA polymerase to reconstruct the gene from its polypeptide product.

    E) DNA ligase to put together fragments of the DNA that codes for a particular polypeptide

    C) reverse transcriptase to reconstruct the gene from its mRNA.

  • feldman2009
    Answered in Chapter 20

    A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics. A eukaryotic protein can be made in bacteria by inserting the gene encoding the protein into a

    a. restriction plasmid.

    b. protein plasmid.

    c. yeast artificial chromosome (YAC).

    d. expression vector.

    e. PCR vector.

    D) they are eukaryotic cells

  • feldman2009
    Answered in Chapter 20

    The DNA fragments making up a genomic library are generally contained in

    A) recombinant plasmids of bacteria.

    B) recombinant viral RNA.

    C) individual wells.

    D) DNA-RNA hybrids

    E) radioactive eukaryotic cells

    A) recombinant plasmids of bacteria.

  • feldman2009
    Answered in Chapter 20

    How does a genomic library differ from a cDNA library?

    A) A genomic library contains only noncoding sequences, whereas a cDNA library contains only coding sequences.

    B) A genomic library varies, dependent on the cell type used to make it, whereas the content of a cDNA library does not.

    C) A genomic library can be made using a restriction enzyme and DNA ligase only, whereas a cDNA library requires both of these as well as reverse transcriptase and DNA polymerase.

    D) The genomic library can be replicated but not transcribed.

    E) The genomic library contains only the genes that can be expressed in the cell.

    C) A genomic library can be made using a restriction enzyme and DNA ligase only, whereas a cDNA library requires both of these as well as reverse transcriptase and DNA polymerase.

  • feldman2009
    Answered in Chapter 20

    Yeast artificial chromosomes contain which of the following elements?

    A) centromere only

    B) telomeres only

    C) origin of replication only

    D) centromeres and telomeres only

    E) centromere, telomeres, and an origin of replication

    E) centromere, telomeres, and an origin of replication
  • feldman2009
    Answered in Chapter 20

    Which of the following best describes the complete sequence of steps occurring during every cycle of PCR?

    1. The primers hybridize to the target DNA.

    2. The mixture is heated to a high temperature to denature the double stranded target DNA.

    3. Fresh DNA polymerase is added.

    4. DNA polymerase extends the primers to make a copy of the target DNA.

    A) 2, 1, 4

    B) 1, 3, 2, 4

    C) 3, 4, 1, 2

    D) 3, 4, 2

    E) 2, 3, 4

    A) 2, 1, 4

  • feldman2009
    Answered in Chapter 20

    A researcher needs to clone a sequence of part of a eukaryotic genome in order to express the sequence and to modify the polypeptide product. She would be able to satisfy these requirements by using which of the following vectors?

    A) a bacterial plasmid

    B) BAC to accommodate the size of the sequence

    C) a modified bacteriophage

    D) a human chromosome

    E) a YAC with appropriate cellular enzymes

    E) a YAC with appropriate cellular enzymes
  • feldman2009
    Answered in Chapter 20

    A student wishes to clone a sequence of DNA of ~200 kb. Which vector would be appropriate?

    A) a plasmid

    B) a typical bacteriophage

    C) a BAC

    D) a plant virus

    E) a large polypeptide

    C) a BAC

  • feldman2009
    Answered in Chapter 20

    The first cell whose entire genome was sequenced was which of the following?

    A) H. influenzae in 1995

    B) H. sapiens in 2001

    C) rice in 1955

    D) tobacco mosaic virus

    E) HIV in 1998

    A)H. influenzaein 1995

  • feldman2009
    Answered in Chapter 20

    Sequencing an entire genome, such as that of C. elegans, a nematode, is most important because

    A) it allows researchers to use the sequence to build a "better" nematode, resistant to disease.

    B) it allows research on a group of organisms we do not usually care much about.

    C) the nematode is a good animal model for trying out cures for viral illness.

    D) a sequence that is found to have a particular function in the nematode is likely to have a closely related function in vertebrates.

    E) a sequence that is found to have no introns in the nematode genome is likely to have acquired the introns from higher organisms
    D) a sequence that is found to have a particular function in the nematode is likely to have a closely related function in vertebrates.
  • feldman2009
    Answered in Chapter 20

    To introduce a particular piece of DNA into an animal cell, such as that of a mouse, you would find more probable success with which of the following methods?

    A) the shotgun approach

    B) electroporation followed by recombination

    C) introducing a plasmid into the cell

    D) infecting the mouse cell with a Ti plasmid

    E) transcription and translation

    B) electroporation followed by recombination