Free Flashcard From Chapter 20
Question:
A principal problem with inserting an unmodified mammalian
gene into a bacterial plasmid, and then getting that gene expressed in
bacteria, is that
A) prokaryotes use a different genetic code from that of
eukaryotes.
B) bacteria translate polycistronic messages only.
C) bacteria cannot remove eukaryotic introns.
D) bacterial RNA polymerase cannot make RNA complementary to
mammalian DNA.
E) bacterial DNA is not found in a membrane-bounded nucleus
and is therefore incompatible with mammalian DNA.
Answer:
C) bacteria cannot remove eukaryotic introns.