Chapter 20
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Chapter 20
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Free Flashcard From Chapter 20
Question:
What is the most logical sequence of steps for splicing
foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform
bacteria with recombinant DNA molecule.
II. Cut
the plasmid DNA using restriction enzymes.
III. Extract
plasmid DNA from bacterial cells.
IV. Hydrogen-bond
the plasmid DNA to nonplasmid DNA fragments.
V. Use
ligase to seal plasmid DNA to nonplasmid DNA.
A) I, II, IV,
III, V
B) II, III, V,
IV, I
C) III, II, IV,
V, I
D) III, IV, V, I,
II
E) IV, V, I, II,
III
Answer:
C) III, II, IV, V, I