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Chapter 20

Question:

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?

I. Transform bacteria with recombinant DNA molecule.

II. Cut the plasmid DNA using restriction enzymes.

III. Extract plasmid DNA from bacterial cells.

IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments.

V. Use ligase to seal plasmid DNA to nonplasmid DNA.

A) I, II, IV, III, V

B) II, III, V, IV, I

C) III, II, IV, V, I

D) III, IV, V, I, II

E) IV, V, I, II, III

Answer:

C) III, II, IV, V, I

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Last update by mayur on 03/27/2014
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Chapter 20
Chapter 20
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Question: What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? I. Transform bacteria with recombinant DNA molecule. II. Cut the plasmid DNA using restriction enzymes. III. Extract plasmid DNA from bacterial cells. IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments. V. Use ligase to seal plasmid DNA to nonplasmid DNA. A) I, II, IV, III, V B) II, III, V, IV, I C) III, II, IV, V, I D) III, IV, V, I, II E) IV, V, I, II, III
Answer: C) III, II, IV, V, I